Figure 2.
MyD88-GFP puncta assembly at clusters of receptor-bound IL1β. (A) Time-lapse TIRF images of MyD88-GFP showing the formation and coalescence of MyD88-GFP puncta at the cell surface. (B) Formation of MyD88-GFP puncta in individual cells (colors). Time = 0 is defined as the point of image acquisition. (C) TIRF images of IL1β-JF646 and MyD88-GFP showing Myddosome formation at clusters of IL1β-bound IL1R. Regions of interest (blue boxes) show three IL1β-receptor clusters (labeled 1–3). Fluorescence intensity time series from the IL1β-JF646 and MyD88-GFP of the three IL1β clusters are shown on the right.

MyD88-GFP puncta assembly at clusters of receptor-bound IL1β. (A) Time-lapse TIRF images of MyD88-GFP showing the formation and coalescence of MyD88-GFP puncta at the cell surface. (B) Formation of MyD88-GFP puncta in individual cells (colors). Time = 0 is defined as the point of image acquisition. (C) TIRF images of IL1β-JF646 and MyD88-GFP showing Myddosome formation at clusters of IL1β-bound IL1R. Regions of interest (blue boxes) show three IL1β-receptor clusters (labeled 1–3). Fluorescence intensity time series from the IL1β-JF646 and MyD88-GFP of the three IL1β clusters are shown on the right.

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