Additional coexpression analysis of centrobin regulation by TRIM37. (A–C) Results of an independent experiment assessing the effect of coexpressing TRIM37 (WT versus Lig^mut) and centrobin on centrobin levels (A) and relative solubility (B). In addition, interaction of Lig^mut TRIM37 with centrobin was analyzed (C). Quantification of levels and relative solubility is shown in Fig. 8 E. (D) Interaction of WT or Lig^mut TRIM37 with centrobin was analyzed in MG132-treated cells using the protocol schematized on the top. Anti-Myc and Anti-FLAG immunoblotting of anti-Myc immunoprecipitates is shown on the bottom. A plasmid encoding HA-ubiquitin was included in the coexpression. α-Tubulin serves as a loading control in A, B, and D.