Centrobin is required to form the condensate but not centrin-containing foci in TRIM37Δ cells. (A) Localization of centrobin and PLK4 in the indicated conditions; centrosomes (white arrowheads) and condensates (yellow arrow) are indicated. The strategy to inducibly knock out CNTROB (iCNTROB KO) and efficacy of the knockout are shown in Fig. S3 A. Graph on the right plots frequency of condensates with and without addition of doxycycline to induce CNTROB KO. Scale bars, 5 µm and 1 µm (insets). Experiment was performed three times with similar results; data shown are from one experiment. (B) Localization of PLK4 and centrin in the indicated conditions; centrosomes (white arrowheads) and condensates (yellow arrow) are indicated. Graphs below plots the frequency of cells with centrin foci (left) and the number of centrin foci per cell (right). Scale bars, 5 µm and 1 µm (insets). Since iCNTROB KO is not 100% efficient, only cells that lacked centrosomal centrobin were quantified in the (+) condition in A and B. The experiment was performed once. (C) Schematics summarizing independent suppression of the condensate and centrin foci by TRIM37.