Condensate-based ectopic spindle poles cause chromosome missegregation. (A) Fixed analysis of chromosome segregation in anaphase-to-telophase stage cells. Left: Images of multipolar segregation and lagging chromosomes; the condensate (arrows) is marked. Graph on the right plots the frequency of these events in control and TRIM37Δ RPE1 cells. Scale bar, 5 µm. Pooled data from six independent samples. (B) Analysis of micronuclei frequency. Left: Image of a cell with a micronucleus (arrow). Right: Graph of results from three experiments together with the mean; error bars represent standard deviation, and ntot is the total number of cells scored. Scale bar, 5 µm. (C) FISH analysis of chromosomes 17 and 18 in telophase cells. Left: Example images of different classes of telophase figures observed. Right: Quantification of the different categories. The experiment was performed once. Scale bar, 10 µm.
