Analysis of centrosomal component localization to centrobin condensate-based ectopic spindle poles and microtubule nucleation activity of mitotic condensates following microtubule depolymerization. (A) Analysis of centrosomal component localization in mitotic TRIM37Δ cells with an ectopic condensate-based spindle pole. Scale bars, 5 µm and 1 µm (insets). (B) Microtubule regrowth analysis comparing control RPE1 and TRIM37Δ RPE1 mitotic cells. The protocol used for this analysis is schematized on the top. Cells were plated overnight (ON), chilled on ice for 40 min, and fixed (0 min) or warmed with 37°C medium for 1 min and then fixed (1 min). Example images of prophase and prometaphase/metaphase stage cells for the 0-min and 1-min conditions are shown. In mitotic TRIM37Δ cells, condensates that nucleated microtubules and condensates that did not were both observed; quantification of the ability of condensates in TRIM37Δ cells to nucleate microtubules after 1 min rewarming is plotted below the images. Scale bars, 10 µm and 2 µm (insets). The experiment was performed once.