Condensates exhibit two related types of structural organization and form by budding off of centrosomes. (A) Exp-SIM of condensates in TRIM37Δ cells and in TRIM37Δ cells expressing Lig^mut TRIM37 immunostained for centrobin. Widefield images are shown on the left; black-and-white images are examples of condensates from different cells. Surface rendering of a condensate in a TRIM37Δ cell is also shown. Scale bars: widefield, 20 µm and 2 µm (panels below image); Exp-SIM, 2 µm. (B) STED microscopy images showing the linear, striated condensates. Scale bars, 0.5 µm. Experiment performed three times with similar results. (C) Quantification of condensate morphologies (see A for example images) from the Exp-SIM analysis. n, number of condensates. The experiment shown in A and C was performed twice with similar results; data shown are from one experiment. (D) Quantification of interstripe or interpuncta distance measured using different methods; n, number of measurements. 10th–90th percentile box-and-whiskers plots with outliers are shown. (E) Live imaging of Lig^mut TRIM37-mNG-labeled condensates in a TRIM37Δ cell expressing mRuby-fused MAP4 microtubule-binding domain to label microtubules; the centrosome (cyan arrowhead) and the condensate (yellow arrow) are indicated. Scale bars, 5 µm. Times are in minutes. (F) Summary of timing of condensate splitting from the centrosome in 24 imaged untreated cells (blue arrows) and 23 imaged cells acutely treated with centrinone (red arrows). Time is in hours relative to mitosis of each mother cell. The experiment shown in E and F was performed once; data for each condition was pooled from two replicate wells imaged in parallel.