Figure S1.
Analysis of TRIM37 localization and additional characterization of phenotypes of TRIM37Δ RPE1 cells. (A) Independent experiment analyzing ciliation frequency (left) and quantification of primary cilium length (right). n, number of cells analyzed; median and 95% CI are shown on top of individual data points in the right graph. The experiment was performed twice, the first experiment in triplicate and the second experiment in duplicate, with similar results; ciliation frequency and cilium length quantified from the second experiment are shown here, and ciliation frequency from the first experiment is shown in Fig. 1 E. (B) Immunofluorescence of centrobin and the indicated centrosomal components in USP28Δ and TRIM37Δ RPE1 cells. Scale bars, 5 µm (1 µm in insets). The experiment was performed once. (C) Expanded view of CLEM analysis of the cell shown in Fig. 2 B. Additional serial sections of the condensate and centrosome, a low-magnification EM view, and a higher-magnification view of the condensate are shown along with replication of the images shown in Fig. 2 B. White scale bar, 20 µm. Black scale bars: low magnification view, 2 µm; condensate and centrosome serial sections, 0.5 µm; zoomed-in view of punctate sheet condensate, 0.1 µm. (D) 3D STORM of condensates labeled with centrobin or PLK4. Reference widefield images are on the left. The STORM images are color coded for depth. Scale bars, 10 µm (widefield), 0.5 µm (STORM). Experiment performed twice with similar results; images are from one experiment. Red arrows indicate that the images on the bottom are higher magnification views of the corresponding regions of the images on top. (E) TRIM37-mNG localization at centrosomes visualized using SIM. CEP152 marks the proximal end of centrioles. CEP164 marks the distal end of the mother centriole. TRIM37 is broadly localized to the centrosome; it does not concentrate in a specific subdomain. Scale bars, 1 µm. The experiment was performed twice with similar results; images are from one experiment. mag, magnification. (F) Rare example of condensate fusion observed by live imaging of Ligmut TRIM37-mNG expressed in TRIM37Δ cells. The fused condensate retains an elongated shape. Scale bar, 5 µm.

Analysis of TRIM37 localization and additional characterization of phenotypes of TRIM37Δ RPE1 cells. (A) Independent experiment analyzing ciliation frequency (left) and quantification of primary cilium length (right). n, number of cells analyzed; median and 95% CI are shown on top of individual data points in the right graph. The experiment was performed twice, the first experiment in triplicate and the second experiment in duplicate, with similar results; ciliation frequency and cilium length quantified from the second experiment are shown here, and ciliation frequency from the first experiment is shown in Fig. 1 E. (B) Immunofluorescence of centrobin and the indicated centrosomal components in USP28Δ and TRIM37Δ RPE1 cells. Scale bars, 5 µm (1 µm in insets). The experiment was performed once. (C) Expanded view of CLEM analysis of the cell shown in Fig. 2 B. Additional serial sections of the condensate and centrosome, a low-magnification EM view, and a higher-magnification view of the condensate are shown along with replication of the images shown in Fig. 2 B. White scale bar, 20 µm. Black scale bars: low magnification view, 2 µm; condensate and centrosome serial sections, 0.5 µm; zoomed-in view of punctate sheet condensate, 0.1 µm. (D) 3D STORM of condensates labeled with centrobin or PLK4. Reference widefield images are on the left. The STORM images are color coded for depth. Scale bars, 10 µm (widefield), 0.5 µm (STORM). Experiment performed twice with similar results; images are from one experiment. Red arrows indicate that the images on the bottom are higher magnification views of the corresponding regions of the images on top. (E) TRIM37-mNG localization at centrosomes visualized using SIM. CEP152 marks the proximal end of centrioles. CEP164 marks the distal end of the mother centriole. TRIM37 is broadly localized to the centrosome; it does not concentrate in a specific subdomain. Scale bars, 1 µm. The experiment was performed twice with similar results; images are from one experiment. mag, magnification. (F) Rare example of condensate fusion observed by live imaging of Ligmut TRIM37-mNG expressed in TRIM37Δ cells. The fused condensate retains an elongated shape. Scale bar, 5 µm.

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