Daple-dependent apical MTs organize coordinated ciliary beating. Schematic illustration of the phenotype of Daple-KO and Vangl1-KO MCCs. Daple KO in tracheal MCCs impair planar polarized apical MTs and induce significant defects in BB orientation at the cell level but not at the tissue level, whereas Vangl1-KO MCCs display significant defects in BB orientation at the tissue level. In Vangl1-KO MCCs, two patterns of apical MT localization were detected. In one pattern, the apical MTs tended to accumulate on one side of the AJC, whereas the other pattern did not show one-sided accumulation.