Figure 8.
Single-molecule behavior of Daple along MTs. (A) Schematic illustration of live imaging of single Daple molecules along an immobilized MT using TIRF microscopy. (B) Time-lapse images of the single-molecule assay with 2 nM mEGFP–Daple FL (green) with fluorescently labeled MTs (magenta). Arrowheads indicate mEGFP–Daple FL molecules. (C) Single frame of the single-molecule assay with 10 nM mEGFP–Daple FL (green) with fluorescently labeled MTs (magenta). (D) Single frames of the single-molecule assays with different concentrations (2, 5, and 10 nM) of mEGFP–Daple FL (green) with fluorescently labeled MTs (magenta). (E) Single frames of the single-molecule assays with 10 µM mEGFP–Daple FL, CT, NT, and ΔCT (green) with fluorescently labeled MTs (magenta). (F) Signal intensity of mEGFP–Daple FL, CT, NT, and ΔCT molecules along an MT (n = 15). Lines represent the mean signal intensity with SE. Scale bars represent 5 µm. A.U., arbitrary units.

Single-molecule behavior of Daple along MTs. (A) Schematic illustration of live imaging of single Daple molecules along an immobilized MT using TIRF microscopy. (B) Time-lapse images of the single-molecule assay with 2 nM mEGFP–Daple FL (green) with fluorescently labeled MTs (magenta). Arrowheads indicate mEGFP–Daple FL molecules. (C) Single frame of the single-molecule assay with 10 nM mEGFP–Daple FL (green) with fluorescently labeled MTs (magenta). (D) Single frames of the single-molecule assays with different concentrations (2, 5, and 10 nM) of mEGFP–Daple FL (green) with fluorescently labeled MTs (magenta). (E) Single frames of the single-molecule assays with 10 µM mEGFP–Daple FL, CT, NT, and ΔCT (green) with fluorescently labeled MTs (magenta). (F) Signal intensity of mEGFP–Daple FL, CT, NT, and ΔCT molecules along an MT (n = 15). Lines represent the mean signal intensity with SE. Scale bars represent 5 µm. A.U., arbitrary units.

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