Figure S5.
MT dynamics at the AJC in WT and Daple-KO tracheal MCCs. (A) Representative immunofluorescence images of EB1-mRuby3 (green) and Daple (magenta) in GFP-centrin2–positive WT MTECs expressing EB1-mRuby3 using a lentivirus system. The EB1-mRuby3 signal intensity is represented by the color map. (B) Representative images of EB1-mRuby3 (green) and centrin2 (blue) in Daple-KO GFP-centrin2–positive MTECs expressing EB1-mRuby3 using a lentivirus system. (C) Representative images of EB1-mRuby3 in Daple-KO GFP-centrin2–positive MTECs expressing EB1-mRuby3 using a lentivirus system. The areas (regions 1 and 2) were arbitrarily selected on the opposite sides of the AJC. (D) Time-lapse images for EB1-mRuby3 in regions 1 and 2 of the AJC in MCCs of GFP-centrin2–positive Daple-KO MTECs expressing EB1-mRuby3 using a lentivirus system. These images were taken in the boxed regions of C. Red arrowheads indicate the EB1 comets contacting the AJC. Yellow arrowheads indicate the EB1 comets that did not contact the cell membrane. (E) Representative images of EB1-mRuby3 in Daple-KO GFP-centrin2–positive MTECs expressing EB1-mRuby3 using a lentivirus system in which the I and P regions of the AJCs are shown. (F) Speed of EB1 at the I and P regions in MCCs of Daple-KO MTECs expressing EB1-mRuby3 using a lentivirus system (n = 8 cells). Significant differences were evaluated using two-tailed Mann-Whitney U tests; n.s., P ≥ 0.05. (G) The vertical plane image of mEGFP–α-tubulin in WT MTECs expressing mEGFP–α-tubulin using a lentivirus system. The arrow indicates cilia. (H) Representative images of mEGFP–Daple FL Δ1390–1867 (green), Fzd6 (magenta), and α-tubulin in Daple-KO MTECs expressing mEGFP–Daple FL Δ1390–1867 using a lentivirus system. Arrows indicate the localization of mEGFP–Daple FL Δ1390–1867 and Fzd6 in tracheal MCCs. Scale bars represent 5 µm in A–C and E–H and 1 µm in D.

MT dynamics at the AJC in WT and Daple-KO tracheal MCCs. (A) Representative immunofluorescence images of EB1-mRuby3 (green) and Daple (magenta) in GFP-centrin2–positive WT MTECs expressing EB1-mRuby3 using a lentivirus system. The EB1-mRuby3 signal intensity is represented by the color map. (B) Representative images of EB1-mRuby3 (green) and centrin2 (blue) in Daple-KO GFP-centrin2–positive MTECs expressing EB1-mRuby3 using a lentivirus system. (C) Representative images of EB1-mRuby3 in Daple-KO GFP-centrin2–positive MTECs expressing EB1-mRuby3 using a lentivirus system. The areas (regions 1 and 2) were arbitrarily selected on the opposite sides of the AJC. (D) Time-lapse images for EB1-mRuby3 in regions 1 and 2 of the AJC in MCCs of GFP-centrin2–positive Daple-KO MTECs expressing EB1-mRuby3 using a lentivirus system. These images were taken in the boxed regions of C. Red arrowheads indicate the EB1 comets contacting the AJC. Yellow arrowheads indicate the EB1 comets that did not contact the cell membrane. (E) Representative images of EB1-mRuby3 in Daple-KO GFP-centrin2–positive MTECs expressing EB1-mRuby3 using a lentivirus system in which the I and P regions of the AJCs are shown. (F) Speed of EB1 at the I and P regions in MCCs of Daple-KO MTECs expressing EB1-mRuby3 using a lentivirus system (n = 8 cells). Significant differences were evaluated using two-tailed Mann-Whitney U tests; n.s., P ≥ 0.05. (G) The vertical plane image of mEGFP–α-tubulin in WT MTECs expressing mEGFP–α-tubulin using a lentivirus system. The arrow indicates cilia. (H) Representative images of mEGFP–Daple FL Δ1390–1867 (green), Fzd6 (magenta), and α-tubulin in Daple-KO MTECs expressing mEGFP–Daple FL Δ1390–1867 using a lentivirus system. Arrows indicate the localization of mEGFP–Daple FL Δ1390–1867 and Fzd6 in tracheal MCCs. Scale bars represent 5 µm in A–C and E–H and 1 µm in D.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal