Figure 5.
The Dvl interactive domain, PBM, of Daple is not required for Daple-mediated apical MT organization. (A) Schematic drawing of Daple FL and its GBA motif and PBM. A comparison between the mouse and human Daple amino acid sequences is shown. (B) Representative immunofluorescence images of mEGFP–Daple FL (green), Fzd6 (magenta), and Odf2 (blue; a marker of MCCs) in Daple-KO MTECs expressing mEGFP–Daple FL. mEGFP–Daple FL was expressed using the lentivirus system. Arrows indicate mEGFP–Daple FL and Fzd6 localization in tracheal MCCs. (C) Representative immunofluorescence images of mEGFP–Daple FL (magenta) and α-tubulin (green) in Daple-KO MTECs expressing mEGFP–Daple FL. The intensity of the fluorescence signal for α-tubulin is represented by the color map, where red indicates high intensity and blue indicates low intensity. (D and E) Intensity profiles of α-tubulin along the linear ROI in mEGFP–Daple FL-positive MCCs (orange) and GFP-negative MCCs (blue) in Daple-KO MTECs. The linear ROI was drawn from the opposite side of the Fzd side of the AJC (distance, 0.00) to the Fzd6 side of the AJC (distance, 1.00). (F) Representative immunofluorescence images of mEGFP–Daple FL ΔPBM (green), Fzd6 (magenta), and α-tubulin (blue) in Daple-KO MTECs, in which mEGFP–Daple FL ΔPBM was expressed. Arrows indicate localization of mEGFP–Daple FL ΔPBM and Fzd6 in tracheal MCCs. (G) Representative images of mEGFP–Daple FL ΔPBM (magenta) and α-tubulin (green) in Daple-KO MTECs expressing mEGFP–Daple FL ΔPBM. The intensity of the fluorescence signal for α-tubulin is represented by the color map, where red indicates high intensity and blue indicates low intensity. (H and I) Intensity profiles of α-tubulin along the linear ROI in mEGFP–Daple FL ΔPBM-positive MCCs (orange) and GFP-negative MCCs (blue) in Daple-KO MTECs. The linear ROI was drawn from the opposite side of the Fzd side of the AJC (distance, 0.00) to the Fzd6 side of the AJC (distance, 1.00). Scale bars represent 5 µm.

The Dvl interactive domain, PBM, of Daple is not required for Daple-mediated apical MT organization. (A) Schematic drawing of Daple FL and its GBA motif and PBM. A comparison between the mouse and human Daple amino acid sequences is shown. (B) Representative immunofluorescence images of mEGFP–Daple FL (green), Fzd6 (magenta), and Odf2 (blue; a marker of MCCs) in Daple-KO MTECs expressing mEGFP–Daple FL. mEGFP–Daple FL was expressed using the lentivirus system. Arrows indicate mEGFP–Daple FL and Fzd6 localization in tracheal MCCs. (C) Representative immunofluorescence images of mEGFP–Daple FL (magenta) and α-tubulin (green) in Daple-KO MTECs expressing mEGFP–Daple FL. The intensity of the fluorescence signal for α-tubulin is represented by the color map, where red indicates high intensity and blue indicates low intensity. (D and E) Intensity profiles of α-tubulin along the linear ROI in mEGFP–Daple FL-positive MCCs (orange) and GFP-negative MCCs (blue) in Daple-KO MTECs. The linear ROI was drawn from the opposite side of the Fzd side of the AJC (distance, 0.00) to the Fzd6 side of the AJC (distance, 1.00). (F) Representative immunofluorescence images of mEGFP–Daple FL ΔPBM (green), Fzd6 (magenta), and α-tubulin (blue) in Daple-KO MTECs, in which mEGFP–Daple FL ΔPBM was expressed. Arrows indicate localization of mEGFP–Daple FL ΔPBM and Fzd6 in tracheal MCCs. (G) Representative images of mEGFP–Daple FL ΔPBM (magenta) and α-tubulin (green) in Daple-KO MTECs expressing mEGFP–Daple FL ΔPBM. The intensity of the fluorescence signal for α-tubulin is represented by the color map, where red indicates high intensity and blue indicates low intensity. (H and I) Intensity profiles of α-tubulin along the linear ROI in mEGFP–Daple FL ΔPBM-positive MCCs (orange) and GFP-negative MCCs (blue) in Daple-KO MTECs. The linear ROI was drawn from the opposite side of the Fzd side of the AJC (distance, 0.00) to the Fzd6 side of the AJC (distance, 1.00). Scale bars represent 5 µm.

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