Figure S2.
Daple is a downstream factor of PCP signaling in tracheal MCCs. (A) Representative immunofluorescence images of Vangl1 (green) and ZO-1 (red) in tracheas of WT and Daple-KO adult mice (upper panels) and schematic illustrations of Vangl1 localization (blue; lower panels). These images correspond to the immunofluorescence images shown in Fig. 2 D. Red arrows display Vangl1 localization in tracheal MCCs. (B) Representative immunofluorescence images of Fzd6 (green) and Odf2 (blue) in tracheas of WT and Daple-KO adult mice (upper panels) and schematic illustrations of Fzd6 localization in MCCs (orange; lower panels). These images correspond to the immunofluorescence images shown in Fig. 1 E. Red arrows show Fzd6 localization in tracheal MCCs. (C) Representative immunofluorescence images of Pk2 (green) and Odf2 (blue) in tracheas of WT and Daple-KO adult mice. Yellow arrows indicate the localization of Pk2 in tracheal MCCs. (D) Representative immunofluorescence images of Fzd6 (green) and Odf2 (blue) in tracheas of Vangl1-KO adult mice. Vangl1-KO MCCs are divided into two types: cell-1 (Fzd6 asymmetrical) and cell-2 (Fzd6 symmetrical). Red arrows indicate the localization of Fzd6 at the AJC in tracheal MCCs. (E) Representative immunofluorescence images of Daple (green) and Odf2 (blue) in tracheas of Vangl1-KO adult mice (lower panels). Schematic illustrations of Daple localization in MCCs (orange; lower panels). Vangl1-KO MCCs were divided into two types: cell-1 (Daple asymmetrical) and cell-2 (Daple symmetrical). These images correspond to the immunofluorescence images shown in Fig. 1 F. (F) Ratio of cell-1 (Fzd6 asymmetrical) and cell-2 (Fzd6 symmetrical) in MCCs of Vangl1-KO mouse tracheas (n = 98 cells). The double arrows indicate the direction of the mounted tracheas in A–E. Scale bars represent 5 µm.

Daple is a downstream factor of PCP signaling in tracheal MCCs. (A) Representative immunofluorescence images of Vangl1 (green) and ZO-1 (red) in tracheas of WT and Daple-KO adult mice (upper panels) and schematic illustrations of Vangl1 localization (blue; lower panels). These images correspond to the immunofluorescence images shown in Fig. 2 D. Red arrows display Vangl1 localization in tracheal MCCs. (B) Representative immunofluorescence images of Fzd6 (green) and Odf2 (blue) in tracheas of WT and Daple-KO adult mice (upper panels) and schematic illustrations of Fzd6 localization in MCCs (orange; lower panels). These images correspond to the immunofluorescence images shown in Fig. 1 E. Red arrows show Fzd6 localization in tracheal MCCs. (C) Representative immunofluorescence images of Pk2 (green) and Odf2 (blue) in tracheas of WT and Daple-KO adult mice. Yellow arrows indicate the localization of Pk2 in tracheal MCCs. (D) Representative immunofluorescence images of Fzd6 (green) and Odf2 (blue) in tracheas of Vangl1-KO adult mice. Vangl1-KO MCCs are divided into two types: cell-1 (Fzd6 asymmetrical) and cell-2 (Fzd6 symmetrical). Red arrows indicate the localization of Fzd6 at the AJC in tracheal MCCs. (E) Representative immunofluorescence images of Daple (green) and Odf2 (blue) in tracheas of Vangl1-KO adult mice (lower panels). Schematic illustrations of Daple localization in MCCs (orange; lower panels). Vangl1-KO MCCs were divided into two types: cell-1 (Daple asymmetrical) and cell-2 (Daple symmetrical). These images correspond to the immunofluorescence images shown in Fig. 1 F. (F) Ratio of cell-1 (Fzd6 asymmetrical) and cell-2 (Fzd6 symmetrical) in MCCs of Vangl1-KO mouse tracheas (n = 98 cells). The double arrows indicate the direction of the mounted tracheas in A–E. Scale bars represent 5 µm.

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