Figure S2.
RanGTP gradient is unaltered by O-GlcNAc perturbations. (A) Ran, Rcc1, RanGAP1, and RanGTP immunofluorescence images of cells treated with DMSO, 10 µM OSMI-4, or 10 µM Thiamet-G for 24 h. (B) Mean nuclear and cytoplasmic intensity (cyto. int.) and the nucleus-to-cytoplasm intensity ratio of Ran, Rcc1, RanGAP1, and RanGTP. Data from the same batch were normalized (Norm.) to the median value of the DMSO condition. n > 1,400 cells for each condition. (C) Top: RanGTP immunofluorescence images of cells transfected with mCherry, RanWT-mRFP1, Ran69L-mCherry, RanT24N-mCherry, or Rcc1-HA. Bottom: Scatterplots of RanGTP nucleus-to-cytoplasm intensity ratio vs. RFP or HA level. n > 300 cells for each condition. Black line is the line fit to the data. Shaded gray area represents the 95% confidence interval. Scale bars, 50 µm. ρ, Pearson correlation coefficient; p, P value.

RanGTP gradient is unaltered by O-GlcNAc perturbations. (A) Ran, Rcc1, RanGAP1, and RanGTP immunofluorescence images of cells treated with DMSO, 10 µM OSMI-4, or 10 µM Thiamet-G for 24 h. (B) Mean nuclear and cytoplasmic intensity (cyto. int.) and the nucleus-to-cytoplasm intensity ratio of Ran, Rcc1, RanGAP1, and RanGTP. Data from the same batch were normalized (Norm.) to the median value of the DMSO condition. n > 1,400 cells for each condition. (C) Top: RanGTP immunofluorescence images of cells transfected with mCherry, RanWT-mRFP1, Ran69L-mCherry, RanT24N-mCherry, or Rcc1-HA. Bottom: Scatterplots of RanGTP nucleus-to-cytoplasm intensity ratio vs. RFP or HA level. n > 300 cells for each condition. Black line is the line fit to the data. Shaded gray area represents the 95% confidence interval. Scale bars, 50 µm. ρ, Pearson correlation coefficient; p, P value.

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