Localization of Leep1 in vegetative and developed cells. (A) GFP-Leep1 translocation in response to cAMP stimulation. (B) Quantification of Leep1 translocation (mean ± SD; n = 6 cells from three videos). (C) MS scores for Leep1 at the respective time points. (D) GFP-Leep1 localization during macropinocytosis. (E) RFP-Leep1 colocalizes with PHcrac-GFP at macropinocytic cups. (F) RFP-Leep1 and Pten-GFP exhibit complementary distributions on the plasma membrane. (G) GFP-Leep1 localization during phagocytosis of TRITC-labeled yeast. (H) GFP-Leep1 localization in cells chemotaxing toward cAMP. A micropipette filled with cAMP was placed at the bottom right corner. (I) GFP-Leep1 localization in cells moving along a self-generated folate gradient. Arrows point to front protrusions; arrowheads point to retracting pseudopods. (J) Time-lapse imaging of cells coexpressing GFP-Leep1 and the F-actin marker LimEΔcoil-RFP. Arrowheads point to pseudopod-type protrusions; arrows point to macropinocytic cups. Scale bars = 10 µm.