Figure 4.
Codegradation of SNS (extracellular DNA) and HyLite488-labeled FN (matrix protein) by invadosomes.(a) A podosome forming THP-1 cell was imaged over 60 min on a surface coated with both SNS and HyLite488-labeled FN (Video 2). (b) Fluorescence intensity analysis shows that the degradation of both SNS and FN is starting at a similar time by podosome. (c) Invadopodia forming MTC cell on a surface coated with both SNS and HyLite488-labeled FN (Video 3). (d) Fluorescence intensity analysis shows that only SNS was degraded by invadopodia; no degradation of FN was observed within the time frame of the experiment. (e) THP-1 and MTC with immunostained F-actin on surface, coated with SNS and FN dye. (f) DNase activity and protease activity in invadopodia (in MTC cells) and podosomes (in THP-1 cells). Statistical analysis was based on 20 cells. Error bars represent SD. Scale bars, 10 µm.

Codegradation of SNS (extracellular DNA) and HyLite488-labeled FN (matrix protein) by invadosomes. (a) A podosome forming THP-1 cell was imaged over 60 min on a surface coated with both SNS and HyLite488-labeled FN (Video 2). (b) Fluorescence intensity analysis shows that the degradation of both SNS and FN is starting at a similar time by podosome. (c) Invadopodia forming MTC cell on a surface coated with both SNS and HyLite488-labeled FN (Video 3). (d) Fluorescence intensity analysis shows that only SNS was degraded by invadopodia; no degradation of FN was observed within the time frame of the experiment. (e) THP-1 and MTC with immunostained F-actin on surface, coated with SNS and FN dye. (f) DNase activity and protease activity in invadopodia (in MTC cells) and podosomes (in THP-1 cells). Statistical analysis was based on 20 cells. Error bars represent SD. Scale bars, 10 µm.

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