Identification of the DNase in invadosomes. (a) SNS and F-actin signals in THP-1 cells treated with PI-PLC, which cleaves GPI anchors. (b) SNS signals in THP-1 cells with 20 cells for each data point, showing that PI-PLC significantly reduced the DNase activity in invadosomes. The merged figure consists of SNS signal in green and anti-DNase X signal in magenta. (c) Immunostaining of THP-l cells with anti-DNase X showed good colocalization between anti-DNase X and SNS signal. (d) THP-1 with DNase X gene knockdown (ΔDNase) and the control cell on the SNS surfaces. Podosomes formed normally in both sets of cells. (e) THP-1 with DNase X gene knockdown showed a significant reduction of DNase activity reported by SNS signal. The analysis was based on 20 cells for each data point. All significance values were evaluated by two-tailed tests. **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001. Error bars represent SD. Scale bars, 10 µm.