DNase in podosomes does not degrade ssDNA but degrade dsDNA irrespective of their sequences. THP-1 cells were plated on ssDNA, CpG-free dsDNA (CpG motif potentially stimulates immune response), dsDNA, and DNA–PNA modified surfaces. Podosomes (identified with an actin core) degraded dsDNA regardless of DNA sequences, but no degradation was exhibited on ssDNA or PNA–DNA hybrid. Refer to Materials and methods for the constructs of these DNA and PNA duplexes.