The role of Rab40 in regulating cell–ECM adherance and lamellipdia dynamics. (A and B) Control, FLAG-Rab40b, Rab40b-4A, and Rab40-3KO cell lines were seeded on collagen-coated coverslips and incubated for 90 min. Cells were then fixed and stained using phalloidin–Alexa Fluor 594. Quantification on the right shows the means and SEM derived from three independent analyses. Dots represent individual cells analyzed. (C) Control, FLAG-Rab40b-4A, and Rab40-3KO cell lines were seeded on collagen-coated coverslips. Invadopodia dynamics was then imaged by time-lapse microscopy/DIC. Small line marks the location of quantification shown on the bottom. To visualize the dynamics of the entire lamellipodia, see Videos 5, 6, and 7.