The effect of Rab40 in regulating non-muscle Myosin IIA/B. (A–D) Control, Rab40b-4A, and Rab40-3KO cell lines were plated on collagen-coated coverslips, fixed and stained using phalloidin–Alexa Fluor 594 and anti-nonmuscle Myosin IIA/B antibodies. Boxes mark the region that is displayed as higher-resolution image. Arrows point to stress fibers. Quantification in D shows the means and SEM derived from three independent analyses. Dots represent individual cells analyzed. (E) Quantification of number of FAs per cell for control, Rab40b-3KO-1, and Rab40b-3KO-2 cells. n ≥ 20 cells per condition. NMY, nonmuscle Myosin.