Rab40b regulates subcellular localization of EPLIN-β. (A) Control or FLAG-Rab40b-4A–expressing cells were transfected with either GFP-EPLIN-α (green) or GFP-EPLIN-β (green). Cells were then fixed and stained with phalloidin–Alexa Fluor 594 (red). Line indicates the area analyzed by line scan and shown in B. (B and C) EPLIN and actin distribution in lamellipodia was analyzed by line scan. The location of line-scan analysis is shown in A. Panel C shows the analysis of the distance between actin front at the leading edge and EPLIN front. The data shown are the means and SEM derived from five different cells. (D) Representative images of immunohistological stains of EPLIN from tumors grown from each cell line. AU, arbitrary units.