Rab40a/b/c depletion or Rab40b-4A overexpression leads to increase in plasma membrane– and stress fiber–associated EPLIN. (A–C) IF images of control (A), FLAG-Rab40b-4A (B), and Rab40-3KO (C) cells plated on collagen-coated coverslips and then fixed and stained with anti-EPLIN antibodies (green) and phalloidin–Alexa Fluor 594 (red). Boxes mark regions of interest. Arrows in whole-field images indicate the lamellipodia leading edge. Arrows in boxed regions indicate stress fibers. (D) Quantification of EPLIN fluorescence in control, FLAG-Rab40b-4A, and Rab40-3KO cells. The data shown are the means and SEM derived from three different experiments. Dots represent individual cells analyzed.