Figure S2.
FA maturation analysis. (A) Images of control and FLAG-Rab40b-4A cells stained with zyxin (green) and paxillin (red). Boxes mark regions that are shown as higher-resolution images. (B) Quantification of percentage of zyxin-positive FAs in control and FLAG-Rab40b-4A cells. n ≥ 15 cells per cell line were analyzed. Control, 76.2 ± 2.9 SEM; FLAG-Rab40b-4A, 89.7 ± 2.1 SEM. (C) WB images of cell lysates blotted with anti-FAK, anti-Y397 pFAK, or anti-S910 pFAK antibodies. Numbers shown are the average densitometry analysis derived from three independent experiments relative to tubulin and standardized to control levels. Control::FLAG-Rab40b, S910 P = 0.05; Control::FLAG-Rab40b-4A, S910 P < 0.005, Y397 P < 0.05.

FA maturation analysis. (A) Images of control and FLAG-Rab40b-4A cells stained with zyxin (green) and paxillin (red). Boxes mark regions that are shown as higher-resolution images. (B) Quantification of percentage of zyxin-positive FAs in control and FLAG-Rab40b-4A cells. n ≥ 15 cells per cell line were analyzed. Control, 76.2 ± 2.9 SEM; FLAG-Rab40b-4A, 89.7 ± 2.1 SEM. (C) WB images of cell lysates blotted with anti-FAK, anti-Y397 pFAK, or anti-S910 pFAK antibodies. Numbers shown are the average densitometry analysis derived from three independent experiments relative to tubulin and standardized to control levels. Control::FLAG-Rab40b, S910 P = 0.05; Control::FLAG-Rab40b-4A, S910 P < 0.005, Y397 P < 0.05.

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