Figure 3.
Rab40b–Cullin5 regulates chemotactic migration and cell invasion.(A and B) Matrigel invasion assay of control, FLAG-Rab40b, and FLAG-Rab40b-4A cells. Representative field-of-view images of Calcein-stained cells at 5-µm increments throughout the plug. (B) Quantification of cell migration through Matrigel plug. Results are of three separate runs, with at least three fields-of-view per condition per run. (C and D) Control, FLAG-Rab40b, or FLAG-Rab40b-4A cells plated on collagen-coated coverslips and then fixed and stained with phalloidin–Alexa Fluor 594 (red), cortactin (green), and DAPI (blue). Inset regions of interest highlight phalloidin/cortactin dual-positive puncta. Arrows point to actin/cortactin puncta. (D) Quantification of dual-positive actin/cortactin puncta. n ≥ 18 cells per condition.

Rab40b – Cullin5 regulates chemotactic migration and cell invasion. (A and B) Matrigel invasion assay of control, FLAG-Rab40b, and FLAG-Rab40b-4A cells. Representative field-of-view images of Calcein-stained cells at 5-µm increments throughout the plug. (B) Quantification of cell migration through Matrigel plug. Results are of three separate runs, with at least three fields-of-view per condition per run. (C and D) Control, FLAG-Rab40b, or FLAG-Rab40b-4A cells plated on collagen-coated coverslips and then fixed and stained with phalloidin–Alexa Fluor 594 (red), cortactin (green), and DAPI (blue). Inset regions of interest highlight phalloidin/cortactin dual-positive puncta. Arrows point to actin/cortactin puncta. (D) Quantification of dual-positive actin/cortactin puncta. n ≥ 18 cells per condition.

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