Cell migration analysis. (A) Table listing other proteins (in addition to Fig. 1) identified by mass spectroscopy analysis from FLAG-Rab40b pull-down. (B) Rates of cell proliferation of control and FLAG-Rab40b-4A cells on either collagen or fibronectin (FN). Data represent the means and SEM derived from three different experiments. (C) Average speed calculated from time-lapse analysis of parental (control) and FLAG-Rab40b-4A cells (also see Videos 1 and 2). Control, 0.24 µm/min ± 0.0 SEM, n = 51 cells; FLAG-Rab40b-4A, 0.25 µm/min ± 0.01 SEM, n = 121. (D) MSD calculated from time-lapse analysis of parental (control) and FLAG-Rab40b-4A cells (also see Videos 1 and 2). Data represent means and SEM derived from n = 39 control cells and n = 79 FLAG-Rab40b-4A cells. P = 0.75. For power-law equation fitting to MSD(Δt) = C*Δt^α, where the exponent α is indicative of type of movement: control, MSD(Δt) = 0.8337Δt^1.3212, α = 1.3212; FLAG-Rab40b-4A, MSD(Δt) = 0.6652Δt^1.3743, α = 1.3743. (E) Directionality ratios control and FLAG-Rab40b-4A cells were calculated from time-lapse analysis (also see Videos 1 and 2). Only cells that remained in frame for the duration of the entire time-lapse experiment were analyzed. Directionality at the last time point is 0.38 ± 0.03 SEM for control cells and 0.29 ± 0.02 SEM for FLAG-Rab40b-4A cells. P < 0.05. (F) Migration analysis of control, FLAG-Rab40b, and FLAG-Rab40b-4A cells by scratch assay. Right: Representative images of cells at various time points. Blue boxes designate computer generated boundaries of original scratch border. Left: Quantification of three separate runs, with at least five wells per condition per run.