Figure 1.
Rab40b binds to Cullin5 in SOCS-dependent and GTP-independent manner.(A) Schematic diagram of Rab40b. (B) List of proteins identified in both GDP- and GTPγS-bound FLAG-Rab40b immunoprecipitates from MDA-MB-231 lysate followed by mass spectroscopy analysis. (C) FLAG-Rab40b binding to GST-Cullin5 as analyzed by glutathione bead pull-down assay. Top panel: Coomassie staining. Bottom panel: WB with anti-FLAG. (D) FLAG-Rab40b (WT and 4A mutants) binding to GST-Cullin5 as analyzed by glutathione bead pull-down assay followed by WB with anti-FLAG (bottom blot) and Coomassie stain (top blot). Numbers shown are the average densitometry analysis derived from three independent experiments, standardized to WT FLAG-Rab40b. WT::4A, P < 0.001. (E) Model showing CRL5 E3 ligase complex partners. Ub, ubiquitin.

Rab40b binds to Cullin5 in SOCS-dependent and GTP-independent manner. (A) Schematic diagram of Rab40b. (B) List of proteins identified in both GDP- and GTPγS-bound FLAG-Rab40b immunoprecipitates from MDA-MB-231 lysate followed by mass spectroscopy analysis. (C) FLAG-Rab40b binding to GST-Cullin5 as analyzed by glutathione bead pull-down assay. Top panel: Coomassie staining. Bottom panel: WB with anti-FLAG. (D) FLAG-Rab40b (WT and 4A mutants) binding to GST-Cullin5 as analyzed by glutathione bead pull-down assay followed by WB with anti-FLAG (bottom blot) and Coomassie stain (top blot). Numbers shown are the average densitometry analysis derived from three independent experiments, standardized to WT FLAG-Rab40b. WT::4A, P < 0.001. (E) Model showing CRL5 E3 ligase complex partners. Ub, ubiquitin.

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