Figure S2. The level and distribution of... | Rockefeller University Press

Figure S2.

The level and distribution of Moe is unaffected by not.(A–C) Confocal micrographs showing egg chambers with either WT or not1 GFP-labeled MARCM clones (green) stained with antibodies against Moe (magenta). DNA stained with TO-PRO-3 is in blue. (A) Stage 10 egg chamber; inset shows magnified image of the mosaic BC cluster, with Moe staining in grayscale visible around the periphery of the cluster surrounding both WT and mutant cells. Scale bar is 25 µm (inset, 10 µm). (B and C) Another example of a mosaic not1 BC cluster to illustrate regions selected for quantitation of Moe staining. (B) Regions of outer BC–nurse cell junctions (arrows) were selected adjacent to not1 cells (green outline) or WT cells (purple outline). (C) Example of positioning of line scans through mutant (green line) and WT (purple line) regions of a mosaic cluster. (D) Quantitation of Moe intensity in WT control and not1 clones (as in B) compared with control sibling cells. Error bars indicate SEM in three biological replicates. (E) Dot plots showing quantification of area under curve for back, middle, and front (BMF) of the BC clusters derived from line scans taken through the cluster (e.g., as in C). Error bars indicate SEM of replicates (n ≥ 8). Significance determined by unpaired two-tailed Student’s t test.

The level and distribution of Moe is unaffected by not. (A–C) Confocal micrographs showing egg chambers with either WT or not¹ GFP-labeled MARCM clones (green) stained with antibodies against Moe (magenta). DNA stained with TO-PRO-3 is in blue. (A) Stage 10 egg chamber; inset shows magnified image of the mosaic BC cluster, with Moe staining in grayscale visible around the periphery of the cluster surrounding both WT and mutant cells. Scale bar is 25 µm (inset, 10 µm). (B and C) Another example of a mosaic not¹ BC cluster to illustrate regions selected for quantitation of Moe staining. (B) Regions of outer BC–nurse cell junctions (arrows) were selected adjacent to not¹ cells (green outline) or WT cells (purple outline). (C) Example of positioning of line scans through mutant (green line) and WT (purple line) regions of a mosaic cluster. (D) Quantitation of Moe intensity in WT control and not¹ clones (as in B) compared with control sibling cells. Error bars indicate SEM in three biological replicates. (E) Dot plots showing quantification of area under curve for back, middle, and front (BMF) of the BC clusters derived from line scans taken through the cluster (e.g., as in C). Error bars indicate SEM of replicates (n ≥ 8). Significance determined by unpaired two-tailed Student’s t test.