Figure 2.
Specific SH2-SH3–containing proteins promote LAT cluster formation.(A) TIRF microscopy revealed LAT microcluster formation with different SH2 and SH3 domain proteins. Alexa Fluor 488 LAT at 300 molecules/µm2 was incubated with 250 nM Sos1 (labeled with Alexa Fluor 647) and indicated proteins at 500 nM. Scale bar, 5 µm. (B) Quantification of LAT clustering and membrane recruitment of Sos1. Shown are mean ± SD; n = 3 independent experiments.

Specific SH2-SH3–containing proteins promote LAT cluster formation. (A) TIRF microscopy revealed LAT microcluster formation with different SH2 and SH3 domain proteins. Alexa Fluor 488 LAT at 300 molecules/µm2 was incubated with 250 nM Sos1 (labeled with Alexa Fluor 647) and indicated proteins at 500 nM. Scale bar, 5 µm. (B) Quantification of LAT clustering and membrane recruitment of Sos1. Shown are mean ± SD; n = 3 independent experiments.

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