Depletion of Tws rescues mitotic defects resulting from the loss of Endos function in the cytoplasm. (A) Expression of the indicated proteins was induced, and cells were transfected with dsRNA targeting endogenous Endos (3′UTR), Tws, and/or nontarget control dsRNA. Representative IF images are shown. Arrowheads indicate scattered chromosomes on a bipolar spindle. Scale bar: 5 μm. (B) Quantification of the percentage of mitotic cells with scattered chromosomes on a bipolar spindle (mean ± SD, n = 3). ****, P ≤ 0.0001.