Endos must be in the cytoplasm before NEBD to promote correct mitotic entry. (A and B) Expression of the indicated proteins was induced, and cells were transfected with dsRNA targeting endogenous Endos (3′UTR) or nontarget control dsRNA. Scale bar: 5 μm. Representative IF images are shown (A). Arrowheads indicate scattered chromosomes on a bipolar spindle (as illustrated in panel B). (C) Quantification of the percentage of mitotic cells with scattered chromosomes on a bipolar spindle (mean ± SD, n = 3). ****, P ≤ 0.0001.