Endos and its human orthologues are enriched in the cytoplasm. (A) The specificity of antibodies against Endos is verified by RNAi and WB. (B) IF reveals that Endos is mainly cytoplasmic. Left: Representative images. Scale bar: 5 µm. Right: Quantifications of the cytoplasmic signal. (C) IF reveals that Endos-Flag and Arpp19-Flag are mainly cytoplasmic. Left: Representative images. Scale bar: 20 µm. Right: Quantifications of the cytoplasmic/nuclear ratio of signal intensities. Bars: mean ± SD. N = number of cells quantified.  (D) Subcellular fractionation and WB show the relative amounts of cytoplasmic and nuclear proteins. MEK and histone H3 are controls as cytoplasmic and nuclear proteins, respectively. C, cytoplasmic; N, nuclear.