Sequence alignment of Drosophila Endos with vertebrate orthologues. The central region is the most conserved. It contains the Gwl phosphorylation site Ser68, which is the main determinant for Endos interaction with PP2A-Tws. Endos also engages in a phosphorylation-independent interaction with PP2A-Tws that requires a region upstream of the Gwl phosphorylation site. Residues in blue are positively charged and reported to promote ENSA dephosphorylation by PP2A-B55 (Cundell et al., 2016). *, perfectly conserved residues. Based on an alignment obtained using Align from UniProt. Dm, Drosophila melanogaster; Hs, Homo sapiens; term, terminal; Xl, Xenopus laevis.