Figure S6.
EPH/EPHRIN signaling effects on cortical tension impact tissue morphology.(A) Top: Representative images of EPHB2-GFP (green) cells mixed with EPHB2-GFP-LifeAct-mCherry (magenta) cells in hanging drop cultures at multiple time points during aggregate formation. Bottom: Representative images of EPHB2-GFP-LifeAct-mCherry (green) cells mixed with cells overexpressing EPHRIN-B1-LifeAct-mCherry (magenta) at multiple time points during aggregate formation. Aggregates fully formed by 72 h. Scale bars, 100 µm. (B) Hanging drop aggregates formed by EPH:EPHRIN mixed cultures at 96 and 120 h with the addition of DMSO (controls) or Y27632 and ML7. Morphology changes observed in segregated aggregates are disrupted by inhibition of ROCK and MLCK with Y27632 and ML7. Scale bars, 50 μm.

EPH/EPHRIN signaling effects on cortical tension impact tissue morphology. (A) Top: Representative images of EPHB2-GFP (green) cells mixed with EPHB2-GFP-LifeAct-mCherry (magenta) cells in hanging drop cultures at multiple time points during aggregate formation. Bottom: Representative images of EPHB2-GFP-LifeAct-mCherry (green) cells mixed with cells overexpressing EPHRIN-B1-LifeAct-mCherry (magenta) at multiple time points during aggregate formation. Aggregates fully formed by 72 h. Scale bars, 100 µm. (B) Hanging drop aggregates formed by EPH:EPHRIN mixed cultures at 96 and 120 h with the addition of DMSO (controls) or Y27632 and ML7. Morphology changes observed in segregated aggregates are disrupted by inhibition of ROCK and MLCK with Y27632 and ML7. Scale bars, 50 μm.

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