Figure 7.
EPH/EPHRIN signaling affects tissue morphology.(A) In the left panels, representative images show that EPHB2-GFP (green) cells mixed with EPHB2-GFP-LifeAct-mCherry (magenta) cells in hanging drop cultures for 72 h form circular aggregates. In the right panels, EPHB2-GFP-LifeAct-mCherry (green) cells mixed with EPHRIN-B1-LifeAct-mCherry (magenta) cells in hanging drop cultures for 72 h segregate and form highly tortuous aggregates. Scale bar, 100 µm. (B) HEK293 cell–isolated aggregates formed in hanging drop cultures 72 h after mixing. Morphology changes observed in segregated aggregates are disrupted by inhibition of ROCK and MLCK with Y27632 and ML7. Scale bars, 50 µm.

EPH/EPHRIN signaling affects tissue morphology. (A) In the left panels, representative images show that EPHB2-GFP (green) cells mixed with EPHB2-GFP-LifeAct-mCherry (magenta) cells in hanging drop cultures for 72 h form circular aggregates. In the right panels, EPHB2-GFP-LifeAct-mCherry (green) cells mixed with EPHRIN-B1-LifeAct-mCherry (magenta) cells in hanging drop cultures for 72 h segregate and form highly tortuous aggregates. Scale bar, 100 µm. (B) HEK293 cell–isolated aggregates formed in hanging drop cultures 72 h after mixing. Morphology changes observed in segregated aggregates are disrupted by inhibition of ROCK and MLCK with Y27632 and ML7. Scale bars, 50 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal