Figure S1.
HEK293 cells lacking EPH/EPHRIN signaling do not have high interfacial tension.(A) Schematic of cell:cell contact angle assay set up for both unmixed and mixed conditions. (B) Individual frames from live imaging (Video 4) at 10 min, 4 h, 8 h, and 12 h of live imaging, showing that cell:cell contacts are dynamic over time. Scale bar, 20 µm. (C) Quantification of cell:cell contact angles at 8 h and 12 h after plating. EPHB2:EPHRIN-B1 cell pairs show a decreased contact angle, or increased interfacial tension, over time. (D) Quantification of cell:cell contact angles at 4 h after plating for unmixed EPHRIN-B1, unmixed EPHB2 cells, and mixed EPHB2 cells with WT HEK293 cells, showing that heterotypic cell pairs where no EPH/EPHRIN signaling is occurring do not display increased interfacial tension compared with homotypic cells. Error bars represent mean ± SD. ****, P < 0.0001 (ANOVA followed by Dunnett’s multiple comparison test).

HEK293 cells lacking EPH/EPHRIN signaling do not have high interfacial tension. (A) Schematic of cell:cell contact angle assay set up for both unmixed and mixed conditions. (B) Individual frames from live imaging (Video 4) at 10 min, 4 h, 8 h, and 12 h of live imaging, showing that cell:cell contacts are dynamic over time. Scale bar, 20 µm. (C) Quantification of cell:cell contact angles at 8 h and 12 h after plating. EPHB2:EPHRIN-B1 cell pairs show a decreased contact angle, or increased interfacial tension, over time. (D) Quantification of cell:cell contact angles at 4 h after plating for unmixed EPHRIN-B1, unmixed EPHB2 cells, and mixed EPHB2 cells with WT HEK293 cells, showing that heterotypic cell pairs where no EPH/EPHRIN signaling is occurring do not display increased interfacial tension compared with homotypic cells. Error bars represent mean ± SD. ****, P < 0.0001 (ANOVA followed by Dunnett’s multiple comparison test).

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