Figure 2.
Membrane-associated COPII is stationary compared with cargo vesicle movement and microtubule plus-end polymerization. (A) Comparison between membrane-associated COPII movement and transport of ER-to-Golgi carriers. An inverted brightest pixel projection of 50 images from a 0.66-s interval time-lapse sequence of COS7 cell coexpressing VSVG-YFP (left, and green in merged image) and the COPII subunit Sec24C-mCherry (center, and red in merged image) after a shift to permissive temperature (32°C). See Video 2. Scale bar = 5 µm. (B) Comparison between membrane-associated COPII and microtubule plus-end polymerization using EB3-GFP. An inverted brightest pixel projection of 50 images from a 3.7-s interval time-lapse sequence of COS7 coexpressing EB3-GFP (left, and green in merged image) and the COPII subunit Sec24C-mCherry (center, and red in merged image). See Video 3. Scale bar = 10 µm.

Membrane-associated COPII is stationary compared with cargo vesicle movement and microtubule plus-end polymerization. (A) Comparison between membrane-associated COPII movement and transport of ER-to-Golgi carriers. An inverted brightest pixel projection of 50 images from a 0.66-s interval time-lapse sequence of COS7 cell coexpressing VSVG-YFP (left, and green in merged image) and the COPII subunit Sec24C-mCherry (center, and red in merged image) after a shift to permissive temperature (32°C). See Video 2. Scale bar = 5 µm. (B) Comparison between membrane-associated COPII and microtubule plus-end polymerization using EB3-GFP. An inverted brightest pixel projection of 50 images from a 3.7-s interval time-lapse sequence of COS7 coexpressing EB3-GFP (left, and green in merged image) and the COPII subunit Sec24C-mCherry (center, and red in merged image). See Video 3. Scale bar = 10 µm.

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