Developmentally regulated Atg40 expression drives selective ERphagy in meiosis. (A) Quantification of meiotic staging for cells of the indicated genotypes. ana, anaphase; meta, metaphase; pro, prophase. (B) Western blot using samples from cells of the indicated genotypes expressing Sec63-GFP taken at the indicated times in meiosis. Blots were probed for GFP and hexokinase. (C) Average and SD quantifying free GFP as a proportion of the total GFP signal at 8 h from n = 3 replicates of the experiment in B. P values calculated using Student’s t test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001. (D) Western blot using samples from cells of the indicated genotypes expressing GFP-Atg8 taken at the indicated times in meiosis and probed for GFP and hexokinase. (E) Average and SD quantifying Rtn1-GFP abundance normalized to hexokinase at 8 h in meiosis using n = 8 replicates from the experiment in Fig. 6 A. P value calculated using Student’s t test. Source data are available for this figure: SourceData FS6.