The ER is degraded by autophagy during meiosis. (A) Western blot with samples taken from cells expressing Rtn1-GFP during vegetative exponential growth (veg) or at the indicated time in meiosis probing for GFP and hexokinase (hexo) loading control. (B) Microscopy of cells expressing Rtn1-GFP, Vph1-mCherry, and Atg1-AID and imaged at the indicated times after transfer to SPO. Presence (+) or absence (−) of osTIR is indicated. Cells were treated with 500 µM auxin after 4 h in SPO. Yellow arrowheads indicate pockets of vacuole. (C) Western blot of cells of the same genotypes as in B probing for GFP, V5, and hexo. Cells were treated with 500 µM auxin at the indicated times. (D) Average and SD quantifying free GFP as a proportion of total GFP signal for n = 3 replicates of the experiment in C. P values calculated by Student’s t test. (E) Western blot with samples from cells of the indicated genotypes and expressing Rtn1-GFP and Atg40-3V5 taken at the indicated times after transfer to SPO, probed for GFP, V5, and hexo. (F) Average and SD quantifying free GFP as a proportion of total GFP signal for n = 3 replicates of the experiment in E. P values calculated by Student’s t test. ns, P > 0.05; *, P < 0.05; **, P < 0.01. Source data are available for this figure: SourceData F5.