Reticulons and Lnp1 regulate meiotic ER remodeling. (A) WT and rtn1Δ rtn2Δ yop1Δ cells expressing GFP-HDEL (ER) and Htb1-mCherry imaged at the cell periphery and cell center immediately after transfer to SPO. (B) Time-lapse microscopy of rtn1Δ rtn2Δ yop1Δ cells expressing GFP-HDEL (ER) and Htb1-mCherry imaged every 3 min in meiosis. Min 0 is defined as the time of ER collapse. (C) Time-lapse microscopy of rtn1Δ rtn2Δ yop1Δ cells expressing GFP-Ist2 and mCherry-HDEL (ER) imaged every 30 min in meiosis. Min 0 is defined as the time of ER collapse. Gini score for Ist2 distribution is shown below each time point. (D) Gini quantification for at least n = 4 cells of the indicated genotypes. The average and SD are shown. (E)lnp1Δ cells expressing GFP-HDEL (ER) and Htb1-mCherry imaged immediately following transfer to SPO or during exponential growth in YPD. (F) WT and lnp1Δ cells expressing Htb1-mCherry and the indicated GFP-tagged protein imaged 0 or 6 h after transfer to SPO. (G) Average and SD quantifying ER focus size for at least n = 100 cells of each of the indicated genotypes. P values calculated by Student’s t test. ****, P < 0.0001. (H) Quantification of the number of foci per cell for of least n = 100 cells of the indicated genotypes. P values calculated by Student’s t test. ns, P > 0.05; ***, P < 0.001; ****, P < 0.0001. (I) Average and SD quantifying percentage tetrad formation scored 24 h after transfer to SPO for the indicated genotypes. n = 3 replicates were counted, with ≥100 cells each time. P values calculated by Student’s t test. *, P < 0.05. (J) WT or rtn1Δ rtn2Δ yop1Δ cells expressing Sey1-GFP or Lnp1-GFP at the indicated times following transfer to SPO. Scale bar = 2 µm for all panels.