Additional data supporting findings. (A) Efficiency of B56 depletion related to Fig. 2 A. (B) Regulation of Cdc20 T59 and T70 by PP2A-B56 in a mitotic extract. The experimental setup was similar to Fig. 1 A. The pT59 and pT70 Cdc20 signal normalized to Cdc20 was set to 1 for the condition treated with LxxIxE peptide. (C) Representative images of live cell imaging of Hela cells depleted of endogenous Cdc20 and complemented with indicated forms of YFP-tagged Cdc20. Scale bar = 40 µm. (D) Quantification of cells that exit mitosis in the presence of nocodazole (30 ng/ml) during live cell imaging as in C from three independent experiments. Percentage of cells from three independent experiments (mean and SD indicated). (E) Hela cells depleted of endogenous Cdc20 and complemented with indicated forms of YFP-tagged Cdc20 in the presence of Taxol (25 nmol, when indicated). Time from NEBD to mitotic exit or end of filming from three independent experiments (each dot represents a single cell and n values are shown in the graph, red line indicates mean and standard deviation). DIC, differential interference contrast.