Figure 5.
ch-TOG, CLASP1, and CAMSAP1/2 are critical for γ-tubulin–independent MT generation in interphase.(A) Frequency of MT regrowth after RNAi-mediated depletion of the indicated genes (30 min). RNAi cocktails targeted two or three genes simultaneously for CLASP or CAMSAP, respectively. Luciferase siRNA was used as a negative control. (B–E) γ-Tubulin degron was combined with RNAi of the indicated genes. Images were taken 30 min after nocodazole washout. Cells with γ-tubulin signals are indicated by yellow circles, whereas red-circled cells have no detectable γ-tubulin signals. n values are listed within each graph.

ch-TOG, CLASP1, and CAMSAP1/2 are critical for γ-tubulin–independent MT generation in interphase. (A) Frequency of MT regrowth after RNAi-mediated depletion of the indicated genes (30 min). RNAi cocktails targeted two or three genes simultaneously for CLASP or CAMSAP, respectively. Luciferase siRNA was used as a negative control. (B–E) γ-Tubulin degron was combined with RNAi of the indicated genes. Images were taken 30 min after nocodazole washout. Cells with γ-tubulin signals are indicated by yellow circles, whereas red-circled cells have no detectable γ-tubulin signals. n values are listed within each graph.

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