MT nucleation without γ-tubulin. (A) Flowchart of interphase MT depolymerization-regrowth assay combined with auxin-induced degron. (B) γ-Tubulin-independent MT generation in interphase cells. MTs were depolymerized, except at the centrioles (0 min), followed by induction of regrowth (10–30 min). In the presence of γ-tubulin, MTs start to regrow within 10 min (yellow), whereas it took >10 min in the absence (red). The faint signals after TubG1 depletion represent autofluorescence. TUB, tubulin.