INM is specifically captured in Atg39-containing NE blebs. (A) Cartoon of protein and protein complexes at the NE, including an SPB and NPC. The boxed region is magnified at bottom to show integral INM proteins and truncations of Heh1 and Heh2 that act as INM reporters. Numbers are amino acids. (B) Deconvolved fluorescent micrographs of the indicated GFP fusion proteins under conditions where mCherry-Atg39 expression is repressed (+ glucose [off], left panels) or induced (+ galactose [on], right panels). Merged images of mCherry (magenta) and GFP (green) are also shown. Arrowheads point to NE blebs containing mCherry-Atg39 and INM proteins. Scale bars are 5 μm. (C) Plot of percentage of cells with Atg39 blebs colocalized with indicated NE proteins. Error bars are SD from three independent replicates of 100 cells per replicate. LEM, LAP2/emerin/MAN1.