Atg39 colocalizes with Atg11. (A) Deconvolved fluorescence micrographs of rapamycin- or carrier-alone (DMSO)–treated cells expressing GFP-Atg11 with the indicated mCherry-fusion proteins. Scale bars are 5 μm. The inset (sixth row, bottom right) shows an example of the ∼10% of cells where mCherry-atg39-ΔL colocalized with GFP-Atg11. The scale bar in the inset is 3 μm. (B) Bar chart plotting the percentage of cells with Atg39–Atg11 colocalization in the indicated strains from experiments as in A. At least 50 cells of each genotype were quantified from three independent experiments. Mean and error bars denoting SD are shown. *, P ≤ 0.05 by one-way ANOVA.