Figure 2.
The Atg39 lumenal domain is required for NE targeting and bleb formation.(A) Schematic of Atg39 with Atg8-interacting motif (AIM; light orange), Atg11 binding region (blue), predicted TM helix (gray), and predicted α helices 1–4 (colored ovals). Numbers are amino acids. (B) Deconvolved fluorescence micrographs of the indicated GFP fusion proteins. Numbers indicate amino acid position from A. Scale bars are 5 μm. (C) As in B. (D) Deconvolved fluorescence micrographs of GFP-Atg39 in the indicated strains. Scale bars are 5 μm. (E) Bar chart plotting the quantification of the number of NE blebs per cell in the indicated strains from experiments as in B–D. Genotype of strains indicated by bar color as shown in key. At least 50 cells of each genotype were quantified from three independent experiments. Mean and error bars denoting SD are shown. ****, P ≤ 0.0001 by one-way ANOVA.

The Atg39 lumenal domain is required for NE targeting and bleb formation. (A) Schematic of Atg39 with Atg8-interacting motif (AIM; light orange), Atg11 binding region (blue), predicted TM helix (gray), and predicted α helices 1–4 (colored ovals). Numbers are amino acids. (B) Deconvolved fluorescence micrographs of the indicated GFP fusion proteins. Numbers indicate amino acid position from A. Scale bars are 5 μm. (C) As in B. (D) Deconvolved fluorescence micrographs of GFP-Atg39 in the indicated strains. Scale bars are 5 μm. (E) Bar chart plotting the quantification of the number of NE blebs per cell in the indicated strains from experiments as in B–D. Genotype of strains indicated by bar color as shown in key. At least 50 cells of each genotype were quantified from three independent experiments. Mean and error bars denoting SD are shown. ****, P ≤ 0.0001 by one-way ANOVA.

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