The split-GFP reconstitution of intranuclear GFP fluorescence depends on the N-terminus of Atg39 (supplemental to Fig. 1 ). (A–C) Deconvolved fluorescence micrographs of the indicated GFP^1–10 constructs coexpressed with the nucleoplasmic split-GFP reporter (see inset and Fig. 1 A). GFP^1–10 constructs are expressed behind a GAL1 promoter and are induced by growth in galactose for the indicated times (in hours). GFP (green), mCherry (magenta), and merged images shown. Scale bars are 5 μm.