Telomere NE tethering during mitosis and G1-phase requires Scs2-Siz2 association and Scs2 SUMOylation. (A) Tethering of Tel14L to the NE was examined using epifluorescence imaging. The percentage of the total number of GFP-lacI/Tel14L-LacO256 foci examined that overlapped with NE-associated Sec63-mCherry signal was determined for at least three biological replicates. The graph shows the average values for various strains at the indicated points in the cell cycle. Cell cycle stage was assessed by bud size and nuclear morphology. n = 50 cells/replicate/cell cycle stage. Error bars represent SD. (B) Sir4-V53 binding to chromatin adjacent to Tel6R was assessed by ChIP and qPCR analysis using asynchronous cultures of the indicated strains. Graphs represent at least three biological replicates. Error bars represent SEM. Asterisks: significant change relative to WT using a two-tailed Student’s t test. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. Phos, phosphorylation.
