Association of lipid moieties with membrane-interacting domains of PFTs . (A) Appearance of faint density corresponding to detergent micelle on visualizing anthrax toxin (Electron Microscopy Data Bank accession no. EMD-6224, PDB under accession no. 3J9C) at a high threshold of volume. (B) Appearance of a prominent density corresponding to detergent micelle on visualizing anthrax toxin at a low threshold of volume. (C) Appearance of faint density corresponding to detergent micelle on visualizing lysenin toxin (Electron Microscopy Data Bank accession no. EMD-8015, PDB accession no. 5EC5) at a high threshold of volume. (D) Appearance of a prominent density corresponding to detergent micelle on visualizing lysenin toxin at a low threshold of volume. (E) Appearance of faint density corresponding to lipid bilayer on visualizing VCC at a low threshold of volume. (F) Increasingly prominent density of lipid bilayer observed on consecutively lowering the threshold of viewing VCC. (G) Further lowering the threshold of viewing shows the fully visible lipid bilayer where the rim domain of VCC is embedded in the membrane.