Comparative analysis of the refined cryo-EM model of membrane-bound VCC with the crystal structure and local resolution estimation. (A) Alignment of a single protomer of the real space-refined cryo-EM model (blue) and the crystal structure (gold) reveals nominal displacement in marked amino acid residues. (B) Local resolution estimation of the fully membrane-bound VCC is demonstrated by a cross section of the side view and the top view of the oligomer. (C) Five segments of the N chain of VCC show the quality of side chain fitting. Residues covered are 140–150, 223–235, 316–326, 378–385, and 573–581. (D) N-chain transmembrane β-strand spanning residues 303–321 shows the resolution of the pore-forming element.