Figure S2.
Overview of cryo-EM data processing pipeline and 3D variability analysis of the liposome-embedded VCC particles.(A) 3D reconstruction workflow of VCC embedded in liposomes. See Materials and methods for more details. (B) EM density corresponding to frame 0 of component 1. The dashed box shows the missing density of the β-barrel. Adjacent figure shows an overlay of frame 19 (yellow) with frame 0 (purple). Cross-sectional view of the overlaid maps indicates the direction of flexibility in the VCC particles. (C) Cross-sectional views of the three distinct structural states represent the size heterogeneity of the β-barrel. A blue scale bar is added corresponding to the size of 19 Å, 50 Å, and 64 Å, respectively. CTF, contrast transfer function.

Overview of cryo-EM data processing pipeline and 3D variability analysis of the liposome-embedded VCC particles . (A) 3D reconstruction workflow of VCC embedded in liposomes. See Materials and methods for more details. (B) EM density corresponding to frame 0 of component 1. The dashed box shows the missing density of the β-barrel. Adjacent figure shows an overlay of frame 19 (yellow) with frame 0 (purple). Cross-sectional view of the overlaid maps indicates the direction of flexibility in the VCC particles. (C) Cross-sectional views of the three distinct structural states represent the size heterogeneity of the β-barrel. A blue scale bar is added corresponding to the size of 19 Å, 50 Å, and 64 Å, respectively. CTF, contrast transfer function.

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