Suppression of GFP-Gpc4–labeled protrusions by expression of dnCdc42 partially blocks rescue by endodermal expression of Gpc4. (A–H) Bright-field images of the indicated embryos. Lines with double arrows show the length of the posterior body; lines of the same color are equal in length. (I) Average posterior body length in embryos shown in A–H from two independent experiments (represented by different color symbols), with the number of embryos indicated. Colored P values correspond to differences between the embryos in which the posterior body is marked with lines of the same color. (J and K) Snapshots from confocal time-lapse imaging performed on the indicated embryos (Video 8). Arrows indicate the direction of migration of the endodermal cells. (L) The total number of protrusions, the length of the protrusion, and the percentages of protrusions of different lengths (grouped into 3-µm bins) in each endodermal cell. The number of embryos, cells, and protrusions analyzed is indicated. Data are mean ± SEM. #, P > 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; unpaired Student's t test.